igf free bsa  (Jackson Immuno)

Journal: Thoracic Cancer

Article Title: Establishing a new human lung squamous cell carcinoma cell line, OMUL ‐1, expressing insulin‐like growth factor 1 receptor and programmed cell death ligand 1

doi: 10.1111/1759-7714.15488

Figure Lengend Snippet: (a) Growth factor receptors and growth kinetics; mRNA expression of FGFR1 , FGFR2 , FGFR3 , FGFR4 , EGFR , HER2 , ErbB3 , ErbB4 , VEGFR1 , VEGFR3 , IGF1R , FLT3 , c‐MET , PDGFRa , and PDGFRb . (b) Effects of picropodophyllin, infigratinib, CP‐673451, SU11274, sapitinib, cetuximab, bevacizumab, carboplatin, and paclitaxel on the proliferation of OMUL‐1 cells ( n = 8, respectively). The growth of OMUL‐1 cells is significantly reduced by picropodophyllin. (c) Immunostaining for IGF1R in the subcutaneous tumor. (d) Immunostaining for IGF1R in the primary tumor. IGF1R is positive in almost all tumor cells in primary and subcutaneous tumors in mice. Scale bar = 100 μm.

Article Snippet: They were then blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with an anti‐insulin‐like growth factor 1 receptor (IGF1R) antibody (NB110‐87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Expressing, Immunostaining

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Immunostaining for IGF1R reveals predominant cytoplasmic staining. (B) Immunostaining for PD-L1 reveals predominant cytoplasmic staining. (C) Hematoxylin-eosin staining.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Immunostaining, Staining

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: Patient characteristics.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Adjuvant

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: Multivariate analysis of the relationship between IGF1R expression and PD-L1 expression.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Expressing

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Comparison of smoking index between the IGF1R-positive and negative groups. The IGF1R-positive group had a significantly larger smoking index. (B) Comparison of smoking index between PD-L1-positive and negative groups. The PD-L1-positive group had a significantly larger smoking index. (C) Comparison of maximum tumor diameter between IGF1R-positive and negative groups. The IGF1R-positive group had a significantly larger maximum tumor diameter.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Comparison

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Kaplan-Meier curve depicting RFS for all patients. The IGF1R-positive group exhibited significantly worse RFS. (B) Kaplan-Meier curve depicting OS for all patients. The IGF1R-positive group exhibited significantly worse OS.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques:

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Kaplan-Meier curve depicting RFS in stage 0–1 patients. The IGF1R-positive group exhibited significantly worse RFS. (B) Kaplan-Meier curve depicting OS in stage 0–1 patients. The IGF1R-positive group exhibited significantly worse OS.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques:

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Kaplan-Meier curve depicting RFS in stage 2 patients. No significant difference was observed between the IGF1R-positive and negative groups. (B) Kaplan-Meier curve for OS in stage 2 patients. No significant difference was observed between the IGF1R-positive and negative groups.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques:

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: (A) Kaplan-Meier curve depicting RFS in stage 3 patients. No significant difference in RFS was observed between the IGF1R-positive and negative groups. (B) Kaplan-Meier curve depicting OS in stage 3 patients. No significant difference was observed between the IGF1-positive and negative groups.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques:

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: Multivariable analysis of RFS in stage 0–1 patients.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Adjuvant

Journal: PLOS ONE

Article Title: Insulin-like growth factor 1 receptor expression correlates with programmed death ligand 1 expression and poor survival in non-small cell lung cancer

doi: 10.1371/journal.pone.0297397

Figure Lengend Snippet: Multivariable analysis of OS in stage 0–1 patients.

Article Snippet: Following this, the slides were blocked with 10% normal mouse serum for 10 min at room temperature and incubated overnight at 4°C with the anti-IGF1R antibody (NB110-87052, 1:200; Novus Biologicals LLC, Centennial, CO, USA).

Techniques: Adjuvant